Private Acces  


One Health network for the prevention of vector-borne diseases around the Mediterranean and Sahel regions

> home | resources | publications



10/2022 - ASSESSMENT OF EXPERTISE IN morphological identification of mosquito species (Diptera, Culicidae) using photomicrographs

Accurate identification of insect species is an indispensable and challenging requirement for every entomologist, particularly if the species is involved in disease outbreaks. The European MediLabSecure project designed an identification (ID) exercise available to any willing participant with the aim of assessing and improving knowledge in mosquito taxonomy. The exercise was based on high-definition photomicrographs of mosquitoes (26 adult females and 12 larvae) collected from the western Palaearctic.

More information


03/2022 - First evidence of Crimean-Congo haemorrhagic fever virus circulation in Bosnia and Herzegovina

This is the first report of anti-CCHFV antibodies in sheep from B&H pro-viding the evidence of CCHFV circulation in the country’s sheep population. So far, these findings indicate the circulation of the virus in the westernmost region of the Balkans and point to the potential CCHFV spread further out of this endemic area.



Since its discovery in the African continent in 1937, West Nile virus expansion and invasion into new regions represent a serious concern today for an increasing number of countries worldwide. This study provides an updated overview on the current knowledge of WNV epidemiology in each African country, summarizing available data on incidence of the infection in humans and animals circulating lineages and clades.



12/2021 - Evaluation of the diagnostic capacities for emerging arboviral diseases in the international network MediLabSecure from 2014 to 2018 - Importance of External Quality Assessments

Emerging infectious diseases pose an increasing threat to all nations around the world, including to developed countries. By definition, because they are rare or unknown, public health systems are not well prepared against these emerging diseases. To be fully prepared, countries must have implemented surveillance systems to monitor rare or unusual sanitary events.



One Health represents a valid scientific strategy, but its adoption in plans and policies both at governmental and international level requires aspects of governance, research and training to be addressed, so that the current fragmentation and barriers can be overcome, as these hamper the interaction, collaboration and coordination between sectors and disciplines that are required. 

At present, stand-alone national One Health plans are often developed. However, in order to benefit from this approach, efforts should be made to appropriately integrate One Health strategies into relevant national and international plans.


10/2021 - Intensive West Nile Virus Circulation in Serbia in 2018 - Results of Integrated Surveillance Program

The WNV surveillance program encompassed the entire territory of Serbia and was conducted by the veterinary service in collaboration with entomologists and ornithologists. The objective of the program was early detection of WNV circulation in the environment and timely reporting to the public health service and local authorities to increase clinical and mosquito control preparedness. 


07/2021 - Whole Genome Sequencing and Phylogenetic Analysis of 6 SARS-CoV-2 Strains Isolated during COVID-19 Pandemic in Tunisia

In Tunisia a first SARS-CoV-2 confirmed case was reported in March 03, 2020. Since then, an increase of cases number was observed from either imported or local cases. The aim of this preliminary study was to better understand the molecular epidemiology and genetic variability of SARS-CoV-2 viruses circulating in Tunisia and worldwide.


06/2021 - Retrospective Identification of Early Autochthonous Case of Crimean-Congo Hemorrhagic Fever, Spain, 2013

Before this report, 7 autochthonous human cases of Crimean-Congo hemorrhagic fever had been reported in Spain, all occurring since 2016. We describe the retrospective identification of an eighth case dating back to 2013. This study highlights that the earliest cases of an emerging disease are often difficult to recognize.


06/2021 - First Serological Evidence of Crimean-Congo Hemorrhagic fever virus and Rift Valeey Fever Virus in Ruminants IN TUNISIA

Crimean-Congo hemorrhagic fever virus (CCHFV, Nairoviridae family) and Rift Valley fever virus (RVFV, Phenuiviridae family) are zoonotic vector-borne pathogens with clinical relevance worldwide. Our study aimed to determine seroprevalences of these viruses and potential risk factors among livestock (cattle, sheep, and goats) in Tunisia.


05/2021 - Moustiques, distribution et richesse spécifique dans huit pays d’Afrique : Cap-Vert, Mauritanie, Sénégal, Gambie, Mali, Burkina Faso, Niger et Tchad

Mosquitoes (Diptera, Culicidae) are a family of insects of considerable importance in public health. A review of litterature has been made to note their presence/absence in eight African countries. All the results are reported in this new paper entitled "Moustiques, distribution et richesse spécifique dans huit pays d’Afrique : Cap-Vert, Mauritanie, Sénégal, Gambie, Mali, Burkina Faso, Niger et Tchad"


02/2021 - The Reported Few Cases and Deaths of Covid-19 Epidemic in Africa Are Still Data Too Questionable to Reassure About the Future of This Continent

This new paper entitled "The Reported Few Cases and Deaths of Covid-19 Epidemic in Africa Are Still Data Too Questionable to Reassure About the Future of This Continent" opens a discussion on the reliability of COVID-9 datas and draw s

More than 10 months after the first case of COVID-19 in Africa was detected (in Egypt on February 14), prevalence and mortality are still relatively low and, although there are many hypotheses, the reasons remain unclear. Reduced virulence of SARS-CoV-2 in Africa, genetic or trained immunity, and young population are among the main reasons being evaluated



04/2020 - enhancing preparedness for arbovirus infections with a one health approach: the development and implementation of multisectoral risk assessment exercises

This new paper entitled «Enhancing Preparedness for Arbovirus Infections with a One Health Approach: The Development and Implementation of Multisectoral Risk Assessment Exercises" synthetises the methodology of the three Multisectoral Risk Assessments exercises carried out with a One Health approach by the mediLabSecure public health working group during the first phase of the project.

Country representatives of the human and animal virology, medical entomology, and public health sectors involved in the surveillance of vector-borne diseases participated in the exercises, one on West Nile virus, one on Crimean–Congo haemorrhagicfever, and one on Rift Valley fever, assessing the added value of this approach to enhancing cross-sectoral collaboration and collective preparedness to emerging viral infections, as well as improving data collection and data-sharing from different sectors.


«Enhancing Preparedness for Arbovirus Infections with a One Health Approach: The Development and Implementation of Multisectoral Risk Assessment Exercises"

01/2020 - Integrated early warning surveillance: Achilles’ heel of One Health?

Emerging and re-emerging infectious diseases and zoonoses indicate the importance of the One Health approach for early warning. At present, even when surveillance data are available, they are infrequently timeously shared between the health sectors. In this context, the public health working group of the MediLabSecure project published an article entitled "Integrated early warning surveillance: Achilles’ heel of One Health?" This article is based on the investigation of the collection of a set of surveillance indicators able to provide data for the implementation of integrated early warning systems in the 22 MediLabSecure participating countries of the Mediterranean, Black Sea and Sahel regions. To do so,  an online questionnaire was used (covering vector, human, and animal sectors), focusing on seven relevant arboviruses, that was submitted to 110 officially appointed experts. Results show that a certain grade of collection of surveillance data (indicators) is already in place in the MediLabSecure region. Their collection should be strengthened and the gaps on critical indicators addressed (i.e., vector infection rate and wildlife information). Deep studies in promising countries could help to address the problem and would represent the chance to pilot integrated early warning systems using data already being collected and to promote the collection of critical data not yet gathered.


"Integrated early warning surveillance: Achilles’ heel of One Health?"


MDPI and ACS Style
Amato, L.; Dente, M.G.; Calistri, P.; Declich, S.; on behalf of the MediLabSecure Working Group. Integrated Early Warning Surveillance: Achilles′ Heel of One Health? Microorganisms 2020, 8, 84.


06/2019 - towards harmonization of entomological surveillance in the mediterranean area

Vector-borne diseases, and in particular, mosquito-borne viral diseases are prime candidates as (re)emerging diseases and are likely to spread across the Mediterranean area. In this context, the implementation of entomological surveillance is, of utmost importance to strenghten preparedness and response capacities to this threat. The article entitled "Towards harmonization of entomological surveillance in the Mediterranean area" synthetises the roadmap toward the harmonization of entomological surveillance systems in the Mediterranean area regarding the risks of mosquito borne virus transmission by providing comprehensive guidelines for designing effective and sustainable entomological surveillance systems.

Source :

Toward harmonization of entomological surveillance in the Mediterranean aera. Jourdain F, Abdallah M. S, Hamidi A, Bouattour A, Alten B, Faraj C, Roiz D, Petric D, Perez-Ramirez E, Velo E, Günay F, Bosevska G, Salem I, Pajovic I, Maric J, Kanani K, Paronyan L, Dente MG, Picard M, Zgomba M, M'hammed S, Haddad N, Gaidash O, Sukhiashvili R, Declich S, Shaibi T, Sulesco T, Harrat Z, Robert V. (2019)PLos Negl Trop Dis 13(6): e0007314.

02/2019 - IMPLEMENTATION of the one health approach to fight arbovirus infections in the mediterranean & black sea region

The article entitled “Implementation of the One health approach to fight arbovirus infections in the Mediterranean and Black Sea Region : Assessing integrated surveillance in Serbia, Tunisia and Georgia" is a synthesis of MeSA studies that have been carried out by the MediLabSecure Public Health Working Group, a qualitative situational analysis involving human, animal and entomology sectors of vector borne disease surveillance in three countries of the MediLabSecure network.



Implementation of the One health approach to fight arbovirus infections in the meduterranean and Black Sea region : Assessing Integrated Surveillance in Serbia, Tunisia and Georgia. Dente MG, Riccardo F, Bolici F, Colella NA, Jovanovic V, Drakulovic M, Vasic M, Mamlouk H, Maazaoui L, Bejaoui M, Zakhashvili K, Kalandadze I, Imnadze P, Declich S. © 2019 The Authors. Zoonoses and Public Health Published by Blackwell Verlag GmbH

DOI: 10.1111/zph.125

01/2019 - distribution chart for euro-mediterranean mosquitoes (western paleartic region)

A synthetic chart of current knowledge of the mosquito ditrubtion in the Euro-Mediterranean Region has been carried out within the MediLabSecure network. Entitled "Distribution Chart for Euo-Mediterranean mosquitoes (Western Paleartic Region", this article synthesises published records in the Western Palaearctic region in an updated distribution chart.


Distribution Chart for euro-Mediterranean mosquitoes (Western Paleartic region).Robert V, Günay F, Le Goff G, Bousses P, Sulesco T, Khalin A, Medlock JM, Kampen H, Petri
ć D, Schaffner F

Journal of the European Mosquito Control Association 37 : 1 - 28, 2019


MediLabSecure Strategic document on Integrated surveillance and risk assessment for arbovirus infections: Recommandations for enhancing One Health in the mediterranean region

In 2007 the public health institutes and ministries of health of the countries of the Mediterranean Basin and Middle East started to collaborate in the framework of the EpiSouth network to strengthen surveillance and control of threats to health.Since then the collaboration has been consolidated and presentlyalso several laboratories are part of the network named MediLabSecure, on the basis of the European project started in 2014 that aimed at enhancing surveillance and control of arbovirus infections. In four years of the MediLabSecure (2014-2017), the studies and the implemented activities provided relevant results and lessons learned reported and discussed in this strategic document. The main objective is to contribute to the strategies for the prevention and control of arbovirus infections with a One Health approach, by focusing on integrated surveillance and multisectoral risk assessments. We propose a conceptual framework to facilitate the description, comparison and assessment of integrated surveillance systems and we provide recommendations to enhance operationalization of One Health strategies in national health policies and regional contexts

10/2018 - An EQA of medical entomology laboratories in the medilabsecure network

Identification of vectors is of prime importance in the field of medical entomology for both operational and research purposes. An external quality assessment of mosquito identification capacities was carried out within the MediLabSecure Network, which is composed of laboratories located in 19 countries close to the European Union around the Mediterranean and Black seas.

Source: Frédéric Jourdain, Marie Picard, Tatiana Sulesco, Nabil Haddad, Zoubir Harrat, Samer Saleh Sawalha, Filiz Günay, Khalil Kanani, Taher Shaibi, Denys Akhramenko, M’hammed Sarih, Enkelejda Velo, Lusine Paronyan, Igor Pajovic, Chafika Faraj, Irakli Sikharulidze, David Putkaradze, Jelena Maric, Golubinka Bosevska, Elizabeta Janceska, Ali Bouattour, Afrim Hamidi, Kurtesh Sherifi, Bülent Alten, Dušan Petrić and Vincent Robert. Parasites & Vectors, 2018

03/2018 - Strengthening Preparedness for Arbovirus Infections in Mediterranean and Black Sea Countries

In the context of One Health, Integrated Surveillance of human, animal, entomological, and environmental sectors constitute one of the main challenges to strengthen the prevention of, and preparadness against, arbovirus infections.

The article recently published in the International Journal of Environmental  Research and Public Health  analyses the conceptual framework to assess integrated surveillance in order to facilitate the identification and description of operationalized One Health surveillance.

Source :

Dente MG, Riccardo F, Nacca G, Ranghiasci A, Escadafal C, Gaayeb L, Jiménez-Clavero MÁ , Manuguerra JC, Picard M, Fernández-Pinero J, Pérez-Ramírez E, Robert V, Victoir K, Declich  (2018) Strengthening Preparedness for Arbovirus Infections in Mediterranean and Black Sea Countries: A Conceptual Framework to Assess Integrated Surveillance in the Context of the One Health Strategy, International Journal of Environmental  Research and Public Health.



Researchers of the MediLabSecure network have collaboratively mapped arboviruses and their vectors in 16 of the project partner countries in the Balkans, the Middle-East and North Africa.

This work underlines the importance of surveillance and the implementation of adapted control strategies of vector-borne diseases in this Region.

Source: Failloux, A.-B., Bouattour, A., Faraj, C.,Gunay, F., Haddad, N., Harrat, Z., Jancheska E., Kanani K., Kenawy M.-A., KotaM., Pajovic I., Paronyan L., Petric D., Sarih M., Sawalha S., Shaibi T.,Sherifi K., Sulesco T., Velo E., Gaayeb L., Victoir K. & Robert, V. (2017). Surveillance of Arthropod-Borne Viruses and their Vectors in the Mediterraneanand Black Sea Regions within the MediLabSecure Network. Current Tropical Medicine Reports.

Curr Trop Med Rep. 2017;4(1):27-39.doi: 10.1007/s40475-017-0101-y. Epub 2017 Mar 17.



The current Zika virus emergence has highlighted the global need to conduct comprehensive risk assessments and develop preparedness activities against arboviral threats. An article published in BMC Public Health examines how the actions and strategies implemented by the MediLabSecure network contribute to the prevention and control of the Zika virus threat in the Euro-Mediterranean area. 

To cite this article, please use:

Risk of Zika virus transmission in the Euro-Mediterranean area and the added value of building preparedness to arboviral threats from a One Health perspective. Escadafal C, Gaayeb L, Riccardo F, Pérez-Ramírez E, Picard M4, Dente MG, Fernández-Pinero J, Manuguerra JC, Jiménez-Clavero MÁ, Declich S, Victoir K, Robert V. BMC Public Health. 2016 Dec 3;16(1):1219. 

PMID: 27914465. 

DOI: 10.1186/s12889-016-3831-1

04/2016 - MEDILABSECURE strengthening arboviruses integrated surveillance in the Mediterranean and black sea

The article entitled “Strengthening integrated surveillance for arboviruses in the Mediterranean and Black Sea regions in the framework of the One Health approach” has just been published. 

It describes how the MediLabSecure project contributes to the strengthening of One Healthsurveillance in the Mediterranean Basin and Black Sea Regions.

You can read and download this article here.

It is part of a monography (with other articles in English) that can be downloaded from the Italian Society of Tropical Medicine and  Global Health (SIMET) website.

To cite this article please use: 

M. G. Dente, F. Riccardo, G. Nacca, A. Ranghiasci, J-C. Manuguerra, C. Escadafal, M. A. Jimenez-Clavero, E. Perez Ramirez, V. Robert, M. Picard, F. Cherblanc, L. Gaayeb, K.Victoir, S. Declich on behal of the MediLabSecure Network  Strenghtening integrated surveillance for arboviruses in the Mediterranean and Black Sea regions in the framework of the One Health approach


Using this website, you may be prompted to give us personal data and information, which we may ask for to send you information related to Pasteur MediLabSecure.
Pursuant to the General Gata Protection Regulation (GDPR), as long as you provide personal ID credentials, you have the right to access, rectify, change, or erase your personal data, and a right to data portability.
You can also, as long as you have legitimate reason, oppose any or all processing of the data that concerns you, or withdraw any consent you had previously given.
If you wish to opt out, exercise your rights, or pose any kind of question on our processing workflows, simply email at :

This website was created and maintained with the financial support of the European Union.

Its contents are the sole responsibility of  the Institut Pasteur and do not necessarily reflect

the views of the European Union

ContactInstitut Pasteur / @MediLabSecure